Aglycones conversion from glucoside

In a recent work with healthy volunteers, black raspberry anthocyanins could be detected under their hydrolyzed aglycone form in the oral cavity, resulting from the activity of B-glycosidase derived both from bacteria and oral epithelial cells [40]. In the same study, parent anthocyanins and cyanidin-3-glucoside microbiota metabolite, protocatechuic acid, were detected in the saliva. Furthermore, saliva samples contained glucuronidated anthocyanin conjugates, consistent with intracellular uptake and phase II conversion of anthocyanins [40]. 

The synthesis of aesculin (14) from cichoriin (11) has also been studied and the process is similar to that required for the conversion of daphnin (12) into daphnetin 8-glucoside (15), as predicted in the last Report. The enzyme, isolated from Cichorium tissue, catalyses the hydrolysis of (11) to aesculetin and also the transglucosylation from another molecule of (11) to the aglycone, with the formation of (14). The reverse reaction (14) -+ (11) does not occur. Unlike the enzyme from Daphne, this one has two interconvertible com-... 

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