Affinity techniques examples

These properties of CDs allow their extensive use in analytical chemistry. For example, CDs can be used for the separation of enantiomers by high performance liquid chromatography (HPLC) or gas chromatography (GC). In these cases, the chiral stationary phase or mobile phase contains CDs or their derivatives. CDs also can be used as enzyme inhibitors in affinity techniques. In spectrometry, CDs... 

The general principles of weak affinity chromatography (WAC) are discussed in this entry. WAC is a subset of affinity chromatography that makes use of weak interactions for the separation or analysis of chemicals. These conditions create a situation in which isocratic elution can be used to pass an analyte through the colurtm. The theory of WAC is discussed and examples are given of ligands that can be used in this technique. Examples of applications that have made use of WAC are also presented. 

The ELP expression system was compared to the conventional oligohistidme fusion, which is traditionally applied for purification by immobilized metal affinity chromatography (IMAC). Both techniques were shown to have a similar yield of the recombinant protein. The temperature-triggered approach offers a fast and inexpensive nonchromatographic separation with the possibility for larger scale purification. Although the ELP expression system may not be applicable to all types of recombinant proteins, numerous examples have already been shown [40]. 

The endothelin B receptor is an example of characterization of a homogeneous, affinity purified protein (Roos et al., 1998). Significant progress has been made in the development of techniques for more high-throughput identification of phosphorlyation events. Analysis of large sets of phosphorylated proteins is facilitated by the availability of affinity purification methods such as anti-phosphotyrosine or anti-phosphoserine antibodies or metal affinity chromatography (Neubauer and Mann, 1999 Soskic et al., 1999). These methods are not specific to a particular protein but rather are used to fractionate all proteins that are phosphorylated. 

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