Affinity chromatography ligand types

M.Y. Arica, M. Yilmaz, E. Yalcin and G. Bayramoglu, Affinity membrane chromatography relationship of dye-ligand type to surface polarity and their effect on lysozyme separation and purification. J. Chromatogr.B, 805 (2004) 315-323. 

There are many types of affinity ligands that can be used in affinity chromatography. The wide range of ligands available for affinity chromatography makes this method a valuable tool for... 

These adsorbants are based on immobilised ligands, which have a high affinity for a particular analyte (Fig. 15.13). There are examples where antibodies have been raised to an analyte and then bound to the surface of a SPE matrix. Various types of chemistry permit this type of immobilisation and affinity chromatography is well established in biochemistry. With the proliferation with biotechnological products such as therapeutic peptides, the use of these types of columns for extraction may increase since they can be designed to be highly selective for such compounds. 

Enz)mies, inhibitors, cofactors, nucleic acids, hormones or cell chromatography can also be utilized as ligands in bioaffinity chromatography types. Examples of these methods include Receptor Affinity Chromatography and DNA Affinity Chromatography [21]. 

With the advent of affinity chromatography to isolate enzymes [7] it was possible to adapt the method for the isolation of antibodies [8], The purification of many types of anti-carbohydrate antibodies with specificity for carbohydrate residues of macromolecules has been achieved. The antibodies were isolated by affinity chromatography on adsorbents with proper ligands. The immunodeterminants of the antigens have been found to be mono- or disaccharide residues of the antigen. The anti-carbohydrate antibodies with unique properties are useful in a variety of medicinal and technological applications. In this article the emphasis is on polyclonal antibodies specifically the preparation, properties and uses of anti-carbohydrate antibodies. 

A two-column affinity chromatography method, Fig. (20 ), was devised and used to separate the anti-arabic antibodies into two sets by using two types of adsorbents [56], For the preparation of one adsorbent a sample of gum arabic was hydrolyzed in 0.01 M HC1 for 10 min and the acid hydrolyzed gum was coupled to AH-Sepharose 4B. The second adsorbent was synthesized by coupling native gum arabic to AH-Sepharose 4B. The columns were attached in a series with the hydrolyzed gum arabic ligand-column preceding the native gum arabic ligand-column. In order to decrease... 

Given below are examples of preparative and large-scale applications of dye-ligand chromatography which indicate that this type of affinity chromatography may be anticipated as an industrial separation technique in near the future. 

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