Affinity chromatography coupling reactions

The methods for production of such immobilized ligands and for carrying out affinity purification of IgG are essentially similar whatever ligand is used. Sepharose 4B is probably the most widely used matrix for affinity chromatography, but there are other materials available. Activation of Sepharose 4B is usually carried out by reaction with CNBr this can be carried out in the laboratory before coupling or ready-activated lyophihzed Sepharose can be purchased. The commercial product is obviously more convenient than homemade activated Sepharose, but it is more expensive and may be less active. 

The fact that adenosine and its derivatives are azo coupling components is used for immobilizing nicotinamide-adenine nucleotide (NAD ) for affinity chromatography purposes. In 87a NAD is bonded to a matrix through an azo bond. 87a is used for the purilication of dehydrogenase enzymes Butler and coworkers have studied the reactions of arenediazonium ions with pyrroles Pyrroles are known to react readily with diazonium ions under... 

Other types of coupled reactions are those of parallel and cyclic substrate conversion. They, too, are copied from nature. Optimization of these coupled reactions leads to systems approaching the functional parameters of biological receptors. On the other hand, the application of biological receptor proteins themselves for analytical purposes is being intensively studied. Thus, an affinity chromatography column with immobilized receptors has been devised (Ray et al., 1979) and, in 1986, Belli and Rechnitz described the first receptrode . 

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