Coagulation factor affinity chromatography

Amatschek K, Necina R, Hahn R, Schallaun E, Schwinn H, Josic D, and Jungbauer A. Affinity chromatography of human blood coagulation factor VIII on monoUths with peptides from a combinatorial Ubrary. J. High Resol. Chromatogr. 2000 23 47-58. 

Example 1 Robustness of an Affinity Chromatography Step for Purification of a Coagulation Factor... 

Feldman P.A., Bradbury P.I., Williams J.D., Sims G.E., Mcphee J.W., Pinnell M.A., Harris L., Crombie G.I. and Evans D.R. 1994. Large-scale preparation and biochemical characterization of a new high purity factor IX concentrate prepared by metal chelate affinity chromatography. Blood Coagul. Fibrin., 5, 939. 

Phosphorylated dextran derivatives, called phosphodex-trans, possess a strong affinity for K-vitamin-dependent coagulation factors, like factor II or prothrombin. This property was used to separate them by affinity chromatography on phosphodextrans, which interact in a similar way as phospholipids from the ceU membrane. ... 

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