Affinity chromatography as a special case of HPLC

Affinity chromatography differs from other chromatographic modes in that a suitable stationary phase can specifically catch either a single or several 

No column is required for isolation purposes hence affinity chromatography is frequently carried out in open systems, e.g. suction filtering. Classical columns with a hydrostatic eluent feed offer a further possibility. This chapter, however, is confined to a description of separations with high-performance stationary phases (10 pm and below) with which rapid chromatography can be achieved. Very small columns may be used. 

The sample size is restricted only by the bonding capacity of the colunrn. The yields of biologically active protein frequently reach 100%, which means that denaturation and irreversible adsorption are negligibly low in many cases. 

Anti-IgG ligands bond all antibodies of the IgG class specifically. The stationary phase synthesis required for the separation IgG shown in Fig. 16.4 

1 =non-retained proteins this peak contains less than 2% peroxidase  

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