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Affinity chromatography application conditions

Successful application of affinity chromatography requires careful design of experimental conditions. The essential components, which are outlined below, are (1) creation and preparation of a stationary matrix with immobilized ligand and (2) design of column development and eluting conditions. [Pg.100]

Isolation of IgG by affinity chromatography involves application of serum to a column of matrix-bound ligand, washing to remove non-IgG components and elution of IgG by changing the conditions such that the ligand-IgG interaction is disrupted. [Pg.105]


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See also in sourсe #XX -- [ Pg.370 ]




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