Affinity chromatography activated solid supports

Compared with enzymes fewer reports are available on immobilization of antibody (Ab) in sol-gels and their applications in immunosensing. Immobilization of Abs on a solid support was first reported in 1967 [128] and the technology has widespread application in affinity chromatography and other areas. However, the major problem associated with covalent immobilization of antibody on solid surface is partial loss of biological activity due to the random orientation of the asymmetric macromolecules,... 

RNA to be studied can be selectively purified by hybridization on solid supports or in agarose chromatography or centrifugation. Conventional solid phases have included nitrocellulose or activated cellulose and affinity columns. Recently, nylon or, particularly, paramagnetic beads have become very useful since hybridization is much faster, reaches completion rapidly, leaches less capture nucleic acid and they allow convenient washes. 

Affinity Chromatography is performed on a unique stationary phase which has a specific bio-active hgand bonded onto a solid support. It is mostly used with bio-molecules and only the active component of a sample is attracted to the stationary phase (e.g., wheat germ lectin for polysaccharides and soybean trypsin inhibitor for proteases). The remaining chemicals are washed off and... 

A major challenge associated with the forward chemical genetic approach is the identification of the target of the small molecule probe.Affinity chromatography is a common approach to target identification. This approach requires the attachment of the small molecule probe to a solid support the site of the attachment of the linker must be chosen carefully to avoid the ablation of the probe s biological activity. Trapoxin, is a cyclic tetrapeptide whose... 

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