Affinity chromatographic method

Immobilized enzymes may be used in affinity chromatographic methods but their use as catalysts may be in either the production or removal of compounds in chemical processes or as analytical tools. Many substrate assays can be performed using enzymes immobilized on a variety of surfaces, e.g. glass beads, plastic or nylon tubing. Alternatively they may be incorporated into gel or microparticulate layers on dry strips or slides. 

A second method of incorporating phase into the selection process is to immobilize the target. Essentially an affinity chromatographic method, this allows nonbound library constituents to be washed away, leaving the selected compound(s) bound to resin. Eliseev s guanidine resin and Still s peptide-bearing beads, both discussed above in the context of exchange reactions, are examples of these. 

An Affinity Chromatographic Method for the Purification of Some Cellulolytic Enzymes... 

The immobilized ligand is an essential factor that determines the success of an affinity chromatographic method [12]. The method which is used for affinity ligand immobilization is important because actual or apparent activity of the final column can be affected. Decrease in... 

A major advance in the elimination of catalytic site inhomogeneity in new preparations came with the development of an affinity chromatographic method (65) for purifying the enzyme. This method made use of the known high affinity of xanthine oxidase for alloxanthine when the enzyme is in a fully reduced state. By attaching alloxanthine to a polymeric matrix, a selective absorption of active enzyme was achieved. 

NJ Hales, E Clayton. Affinity chromatographic method for screening combinatorial libraries. Patent Appl PCT Int App. W09632642 1995. 

Antibodies to a heteroglycan of Streptococcus bovis have been purified by an affinity chromatographic method. 

Two anti-tumour proteins, abrin A and abrin B, have been isolated from Abrus precatorius by ion-exchange and affinity chromatographic methods. Both proteins have similar molecular weights (6.5 X10 ), exist as dimers, and have similar, but not identical, amino-acid compositions. 4-Labelled abrin A and abrin B bind to Sarcoma 180 cells, but the patterns of inhibition of binding by different sugars are different. 

An affinity chromatographic method developed for the purification of a-L-fucosidase I from almond emulsin was based on modified agarose derivatized with 6-aminohexanoyl-lacto-A-fucopentaosylamine. The final enzyme preparation was free from other glycoside hydrolases. 

General affinity chromatographic method for lectin purification... 

The arylsulphatases A and B of human urine, rat liver, rat brain, and sheep brain have been separated by a simple and rapid affinity chromatographic method on agarose-immobilized Cibacron Blue F3GA. ... 

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