Affinity beads

Holland et al. proposed a simpler and faster approach using the protein profile obtained directly from whole cells rather than from cellular extracts or affinity beads.17 This was the first report of the whole-cell MALDI-TOF MS... 

Shimizu, N., Sugimoto, K., Tang, J., et al. (2000). High-performance affinity beads for identifying drug receptors. Nat. Biotechnol, 18, 877-881. 

Uga, H., Knramoii, C., Ohta, A., et al. (2006). A new mechanism of methotrexate action revealed by target screening with affinity beads. Mol Pharmacol, 70, 1832-1839. 

Ohtsu, Y., Ohba, R., Imamura, Y., et al. (2005). Selective ligand purification using high-performance affinity beads. Anal Biochem., 338, 245-252. 

One of the objectives of a biochemical separation system design is to minimize the number of steps (Figure 1) One way of accomplishing this is to perform the separation and concentration in situ that is directly with the whole fermentation broth using solids phase adsorbents. This type of separation requires the design of an affinity bead that provides for selective product removal from the fermentation broth. 

Figure 7 shows the variation of selectivity with respect to time for three types of affinity beads (Cases (a), (b) and (c)). In all three cases, selectivity decreases from the initial maximum value as time progresses. Due to identical diffusivities, the two products have very similar concentration profiles within the immobilized adsorbent bead at initial time. Thus the initial selectivity is just the ratio of their adsorption rate constants. However, since product... 

Preparation of specific antisera against adeno- 136 viruses by affinity bead immunization Development of an immunoadsorbent method 137 for the continuous purification of human placental alkaline phosphatase Purification by immunoadsorption of a-amylase 138 inhibitor from P. vulgaris... 

Sakamoto, S., Kabe, Y., Hatakeyama, M., Yamaguchi, Y., and Handa, H. (2009) Development and application of high-performance affinity beads toward chemical biology and drug discovery. Chem. Rec., 9, 66-85. 

Add 100 pi (bed volume) calmodulin affinity beads to the supernatant (tee Note 5). 

The binding of CBP to the calmodulin affinity beads is calcium-dependent. Make sure the binding buffer contains 2 mM Ca +. 

One can mention that affinity beads are also being used in batch affinity purification by suspending the beads in... 

Enrichment of OP-Albumin Pepsin Peptides on PHOS-Select Iron Affinity Beads... 

Jiang, W., Dubrovskii, Y.A., Podolskaya, E.P., et al., 2013b. PHOS-select iron affinity beads enrich peptides for the detection of organophosphorus adducts on albumin. Chem. Res. Toxicol. 26 (12), 1917-1925. 

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