Hemoglobin adult

Hb-F can also be separated from Hb-A and many of Its variants by both onion- and caiion-chnomatogAjophy. However, In most Instances Hb-F elutes together with the minor adult hemoglobin Hb-Ai thus preventing Its accurate quantitation. 

Sickle cell trait (SCT) is the heterozygous form (HbAS) of SCD in which a person inherits one normal adult hemoglobin (HbA) gene and one sickle hemoglobin (HbS) gene. These individuals... 

Raghavan and Gonick 1977). Fetal hemoglobin appears to have a higher affinity for lead than adult hemoglobin (Ong and Lee 1980c). 

In another aquatic f rog, Amphiuma, the adult hemoglobin has an acid Bohr effect at physiological pH, converted in vivo to a weak alkaline one... 

In adults, hemoglobin (HbA see below) is a heterotetramer consisting of two a-chains and two p-chains, each with masses of 16 kDa. The a- and p-chains have different sequences, but are similarly folded. Some 80% of the amino acid residues form a-helices, which are identified using the letters A-H. 

Deoxygenated sickle cell hemoglobin (deoxyHbS), the j8-Glu-6-Val point mutant form of adult hemoglobin, appears to obey the following empirical rate law for nucleation of polymerization ... 

Table values were calculated based on a normal adult hemoglobin of 14.8 g/dL for weights greater than 15 kg (33 lbs) and a hemoglobin of 12 g/dL for weights 15 kg (33 lbs) or less. For adults and children older than 15 kg (33 lbs), use the lesser of lean body weioht or actual bodv weioht in kiloorams. See above eouation. ... 

Adult hemoglobin (HbA) is a beterotetramer of two a and two P subunits, each of which has a protein component called globin that has a structure similar to myoglobin. Each subunit also has a heme group with a Fe atom at its center (Figure 2-4). 

A 24-year-old man from Liberia is being treated for malaria with 30 mg daily of primaquine. After 4 days of treatment, he returns with the complaint that he has no energy at all. Blood work indicates that he is severely anemic, and dense precipitates are present in otherwise normal-looking RBCs, which contain normal levels of adult hemoglobin. A week after suspending the primaquine treatment, he reports feeling better and his RBC count returns to normal. 

Sickle hemoglobin (HbS) differs from normal adult hemoglobin (HbA) at amino acid number 6 of the P-globin chain, where HbS has a Val and HbA a Glu. 

Figure 27-10 Organization of the globin genes on human chromosomes 11 and 16. The composition of the various embryonic, fetal, and adult hemoglobins is also indicated. Closed boxes indicate active genes and open boxes pseudogenes. The triangles ( ) indicate Alu repetitive sequences and their orientation. The shaded boxes indicate Kpn repeat sequences and the half-arrows their respective orientation. The Kpn sequence between the e and 7° genes in fact consists of two tandemly linked Kpn repeats. From Karlsson and Nienhuis196 and Proudfoot.197...

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