Adsorptive-bubble separation stripping

By using an anionic collector and external reflux in a combined (enriching and stripping) column of 3.8-cm (1.5-in) diameter with a feed rate of 1.63 ni/n [40 gal/(h ft )] based on column cross section, D/F was reduced to 0.00027 with C JCp for Sr below 0.001 [Shou-feld and Kibbey, Nucl. AppL, 3, 353 (1967)]. Reports of the adsubble separation of 29 heavy metals, radioactive and otheiwise, have been tabulated [Lemlich, The Adsorptive Bubble Separation Techniques, in Sabadell (ed.), Froc. Conf. Traces Heavy Met. Water, 211-223, Princeton University, 1973, EPA 902/9-74-001, U.S. EPA, Reg. 11, 1974). Some separation of N from by foam fractionation has been reported [Hitchcock, Ph.D. dissertation. University of Missouri, RoUa, 1982]. 

Commercially available cellulose acetate sheets have a homogeneous micropore structure. Unlike paper, very little adsorption occurs. Cellulose acetate is less hydrophilic than paper and so less buffer is held in the medium, leading to shorter separation time and better resolution than paper and less sample required than with other mediums. The cellulose acetate sheet must be saturated with buffer prior to electrophoresis. This is done by floating the strip on the surface in a shallow tank, since rapid immersion can trap air bubbles in the medium. The relatively low amount of buffer held by the sheet can lead to excessive heat production and drying out. Cellulose acetate electrophoresis plays an important role in clinical diagnostics, for example, in the analysis of hemoglobin. It is also commonly used in the separation of other blood proteins, enzymes, mucopolysaccharides, urine, and other bodily fluids. 

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