Adoptive T cell transfer

A broad and vigorons T cell response generally accompanies elimination of HBV as well as HCV infection. By contrast, patients with chronic hepatitis B or C tend to have late, transient, or narrow T cell responses. In a long-term follow-up of HBV-infected patients receiving HPC transplants from HBV-immune individuals, 20 of 31 recipients cleared their HBV infection (Hui et al. 2005). In principle, these results encourage the development of adoptive T cell transfer strategies for the treatment of chronic viral hepatitis. However, it is still controversial whether induction of an efficient T cell response is the cause or the consequence of viral clearance. Furthermore, T cell responses do not only contribute to virus control but also to disease pathology (Rehermann and Nascimbeni 2005). 

FIGURE 7.4 (A) Donor CD4+T cell proliferation, as reflected by dilution of the fluorescence associated with CFSE, is not altered by TCDD exposure. Changes in expression ofT cell activation markers (B) CD62L and (C) CD25, are induced by TCDD exposure and are dependent on cell division. Data represent donor CD4+ T cells responding to alloantigen in F1 hosts 48 hours after adoptive transfer. F1 host mice were treated with vehicle or TCDD one day prior to injection of CFSE-labeled donorT cells. Adapted from Funatake et al., 2005. 

FIGURE 7.7 (See color insert) Adoptively transferred D011.10 transgenicT cells can be identified by expression of CD4+ and KJ-126 in spleen cell suspension from Balb/c mice after ovalbumin (OVA) immunization. Balb/c mice were injected iv with D011.10 spleen cells containing 3-5 x 1 06CD4+KJ-126+ cells and immunized by intraperitoneal injection of 2 mg OVA emulsified in complete Freund s adjuvant 2 days later. OVA immunization increases the frequency of KJ+T cells and alters the expression of various surface molecules consistent with T cell (Tc) activation. 

FIGURE 7.7 Adoptively transferred DO11.10 transgenic T cells can be identified by expression of CD4+ and KJ-126 in spleen cell suspension from Balb/c mice after ovalbumin (OVA) immunization. For description, see page 112. 

The suppression of DTH (in vivo) by CD8-I-Tregs affords a direct demonstration of the suppressive effects of these cells on effector T cells because adoptive transfer assays demonstrate a suppressive effect on the DTH response within 24 h of the introduction of CD8-I- Tregs into the challenge site for DTH at the time of challenge with the antigen. The ability of CD8-I- suppressor T cells to suppress directly DTH in vivo has been measured by the local adoptive transfer assay (LAT) that transfers DTH to naive recipients by... 

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