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Adipic acid dihydrazide modification

The following protocols make use of the compounds adipic acid dihydrazide and carbohy-drazide to derivatize molecules containing aldehydes, carboxylates, and alkylphosphates. The protocols are applicable for the modification of proteins, including enzymes, soluble polymers such as dextrans and poly-amino acids, and insoluble polymers used as micro-carriers or chromatographic supports. [Pg.139]

Most proteins contain an abundance of carboxylic acid groups from C-terminal functionalities and aspartic and glutamic acid side chains. These groups are readily modified with bis-hydrazide compounds to yield useful hydrazide-activated derivatives. Both carbohydrazide and adipic acid dihydrazide have been employed in forming these modifications using the carbodi-imide reaction (Wilchek and Bayer, 1987). [Pg.142]

Figure 1.112 Phosphate groups can be modified with adipic acid dihydrazide in the presence of a carbodi-imide to produce hydrazide derivatives. This is a common modification route for the 5 -phosphate group of oligonucleotides. Figure 1.112 Phosphate groups can be modified with adipic acid dihydrazide in the presence of a carbodi-imide to produce hydrazide derivatives. This is a common modification route for the 5 -phosphate group of oligonucleotides.
The activation of enzymes using adipic acid dihydrazide and EDC is identical to the procedure outlined for the modification of (strept)avidin (Chapter 23, Section 5). Alternatively, hydrazide groups may be created on enzymes using the heterobifunctional chemoselective reagents described in Chapter 17, Section 2. [Pg.968]

The following protocol describes the modification of DNA or RNA probes at their 5 -phosphate ends with a bis-hydrazide compound, such as adipic acid dihydrazide or carbohydrazide. A similar procedure for coupling the diamine compound cystamine can be found in Section 2.2 (this chapter). [Pg.980]

Protocols for the use of adipic acid dihydrazide in the modification of aldehyde or... [Pg.243]

Modification of Hib capsular polysaccharide for conjugation, (a) CNBr/OIH (b) Adipic acid dihydrazide (c) Car-bonyldimidazoie/OH- (d) Butanedianine (e) p-nitrophenyibromoacetate (f) Sodium periodate (g) Acetic acid (h) Ethyiendiamine, sodium cyanoborohydride... [Pg.2708]

Protocols for the use of adipic acid dihydrazide in the modification of aldehyde or carboxylate functional groups can be found in Chapter 1, Section 4.5, and Chapter 13, Section 5. [Pg.223]

Figure 8.1 Selective primary modification of carboxyl group on HA. AEMA 2-aminoethyl methacrylate hydrochloride ADH adipic acid dihydrazide APMA 2-aminoethyl methacrylate CDH carbodihydrazide HDMA hexane-1,6-diamine PDPH pyridyldithioethylamine SH thiol and VS vinyl sulfonyl... Figure 8.1 Selective primary modification of carboxyl group on HA. AEMA 2-aminoethyl methacrylate hydrochloride ADH adipic acid dihydrazide APMA 2-aminoethyl methacrylate CDH carbodihydrazide HDMA hexane-1,6-diamine PDPH pyridyldithioethylamine SH thiol and VS vinyl sulfonyl...

See other pages where Adipic acid dihydrazide modification is mentioned: [Pg.140]    [Pg.140]    [Pg.146]    [Pg.141]    [Pg.121]    [Pg.18]    [Pg.50]    [Pg.163]   


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ADIPATE

Adipic acid

Adipic acid dihydrazide

Adipic acid dihydrazide enzyme modification

Adipic acid dihydrazide enzyme modification using

DIHYDRAZIDE

Dihydrazides

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