Adipic acid dihydrazide enzyme modification

The following protocols make use of the compounds adipic acid dihydrazide and carbohy-drazide to derivatize molecules containing aldehydes, carboxylates, and alkylphosphates. The protocols are applicable for the modification of proteins, including enzymes, soluble polymers such as dextrans and poly-amino acids, and insoluble polymers used as micro-carriers or chromatographic supports. 

The activation of enzymes using adipic acid dihydrazide and EDC is identical to the procedure outlined for the modification of (strept)avidin (Chapter 23, Section 5). Alternatively, hydrazide groups may be created on enzymes using the heterobifunctional chemoselective reagents described in Chapter 17, Section 2. 

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