Acetophenone 6-chloro-2-hydroxy-4-methyl

Cassimjee et al. reported [22] that a variant engineered aminotransferase at TrpOOCys from Chromohacterium violaceum showed a 29-fold increase on spedfidty for synthesis of (S)-phenylethylamine and about fivefold on spedfidty for 4 -substituted acetophenones, where the groups at 4 position were bromo, chloro, hydroxy, methoxy, nitro, methyl, and cyano. The amine donor was isopropylamine. The reaction schemes are shown in Figure 7.5. 

Preparation by iodination of 5-chloro-2-hydroxy-4-methyl-acetophenone with iodine (1 mol) and iodic acid (1 mol) in ethanol at 35 0° for 1.5 h (75-85%) [1802]. 

Obtained (by-product) by reaction of m-chloro-perbenzoic acid with 5-allyl-2-hydroxy-4-[6-(methyl-thio)hexyloxy] acetophenone in methylene chloride, first at 0°, then at r.t. (9%) [2678,2679]. 

Obtained by hydrolyzing 2-hydroxy-5-(chloro-methyl)acetophenone [5880,5881]. 

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