Acetobacterium woodii

Another example for the use of hydrogen as reductant is observed in the reduction of imine [5b]. New imine reductase activity has been discovered in the anaerobic bacterium Acetobacterium woodii by screening a dynamic combinatorial library of virtual imine substrates, using a biphasic water-tetradecane solvent system. 

Tschech A, N Pfennig (1984) Growth yield increase linked to caffeate reduction in Acetobacterium woodii. 

Tetrachloroethene can be dechlorinated to trichloroethene as the sole prodnct by the homoacetogen Sporomusa ovata nsing methanol as the electron donor, and cell extracts of other homoacetogens inclnding Clostridiumformicoaceticum and Acetobacterium woodii were able to carry this ont nsing CO as electron donor (Terzenbach and Blant 1994). 

Cultures of a number of anaerobic bacteria are able to dechlorinate tetrachloromethane and Acetobacterium woodii formed dichloromethane as the final chlorinated metabolite by successive dechlorination, although CO2 was also produced by an unknown mechanism (Egli et al. 1988). 

Egli C, T Tschan, R Scholtz, AM Cook, T Leisinger (1988) Transformation of tetrachloromethane to dichloro-methane and carbon dioxide by Acetobacterium woodii. Appl Environ Microbiol 54 2819-2824. 

Hasham SA, DL Freedman (1999) Enhanced biotransformation of carbon tetrachloride by Acetobacterium woodii upon addition of hydroxycobalamin and fructose. Appl Environ Microbiol 65 4537-4542. 

Bache R, N Pfennig (1981) Selective isolation of Acetobacterium woodii on methoxylated aromatic acids and determination of growth yields. Arch Microbiol 130 255-261. 

Muller V, Aufurth S, Rahlfs S. 2001. The Na -cycle in Acetobacterium woodii identification and characterization of a Na -translocating FiFo-ATPase with a mixed oligomer of 8 and 16-kDa proteolipids. Biochim Biophys Acta 1505 108-20. 

Aufurth S, Schagger H, Muller V. 2000. Identification of subunits a, b, and cl from Acetobacterium woodii Na -FiFo-ATPase. Subunits cl, c2, and c3 constitute a mixed c-oligomer. J Biol Chem 275 33297-301. 

Heise R, Muller V, Gottschalk G. 1992. Presence of a Na -translocating ATPase in membrane vesicles of the homoacetogenic ha.cXermm Acetobacterium woodii. Eur J Biochem 206 553-7. 

Redlinger J, Muller V. 1994. Purification of ATP synthase from Acetobacterium woodii and identification as a Na -translocating FiFq type enzyme. Fur J Biochem 223 275-83. 

Ragsdale, S. W., and Ljungdahl, L. G., 1984, Hydrogenase from Acetobacterium woodii. Arch. Microbiol. 139 3619365. 

The A/iNa formed may be converted via a Na /H antiporter into a AftfT which then drives the synthesis of ATP via a DCCD-sensitive H -translocating ATP synthase. This ATP formation explains net ATP synthesis coupled to acetate formation from H2/CO2 [192,195,199], Alternatively, A/lNa" could drive ATP synthesis directly via Na "-translocating ATP synthase. A Na -stimulated ATP-synthase activity has recently been reported for Acetobacterium woodii [200]. 

Altered Aromatic Metabolites. Loss of parent compound is insufficient evidence to support the occurrence of biodegradation. Frequently, the disappearance of the parent aromatic compound results in the formation of an altered aromatic metabolite. This product indicates that, although biotransformation has taken place, biodegradation has not. An example is the anaerobic O-demethylation of chlorinated guaiacols to chlorocatechols mediated by the acetogenic bacteria, Acetobacterium woodii and Eubacterium limosum (37). 

Acetobacterium woodii (strain WB1), Clostridium methoxybenzovorans (strain SR3) and Eubacterium callanderi (strain FD) were from the laboratory collection of LOMA-IRD (Marseille, France). Enterobacter cloacae (strain DG6) (ATCC... 

Hydrogenases are found in a wide variety of anaerobic bacteria, such as the eubacterial C. pasteurianum and Acetobacterium woodii and the archaebacterial... 

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