Sporomusa ovata

Tetrachloroethene can be dechlorinated to trichloroethene as the sole prodnct by the homoacetogen Sporomusa ovata nsing methanol as the electron donor, and cell extracts of other homoacetogens inclnding Clostridiumformicoaceticum and Acetobacterium woodii were able to carry this ont nsing CO as electron donor (Terzenbach and Blant 1994). 

Stupperich E, R Konle (1993) Corrinoid-dependent methyl transfer reactions are involved in methanol and 3,4-dimethoxybenmzoate metabolism by Sporomusa ovata. Appl Environ Microbiol 59 3110-3116. 

Stupperich, E., Eisinger, H. J., and Albracht, S. P. J., 1990, Evidence for a super-reduced cobamide as the major corrinoid fraction in vivo and a histidine residue as a cobalt ligand of the para-cresolyl cobamide in the acetogenic bacterium Sporomusa-ovata, Eur. J. Biochem. 193 1050109. 

Stupperich, E. Konle, R. Lehle, M. Corrinoid-dependent Methyl Transfer Reactions in Sporomusa ovata. In Vitamin Biz tid Biz Pfoteins Krautler, B., Arigoni, D., Golding, B. T, Eds. Wiley-VCH Weinheim, 1998 pp 179-187. 

The crystallographic results on the structure of MetH and the finding of the base-off/His-on binding of the cofactor in a Bi2-dependent methyl transferase were consistent with earlier ESR spectroscopic evidence for histidine binding to the cobalt center of p-cresolyl-cobamide (52) in the aceto-gen Sporomusa ovata [145,169]. Various other B -dependent methyltrans-ferases are indicated to have either a base-off/His-on bound methyl-Co(III)-corrinoid, or even a methyl-corrinoid cofactor in base-off form (where His-coordination is absent) [156]. 

Sporomusa ovata 2 CO2 + 2 H2O CH3COOH + 2 O2, oxobutyrate is produced as side product H-cell, - 400 mV versus NHE, unpolished graphite, 86 % ECE, acetate 153 pmol/day, 2-oxobutyrate 6.7 pmol/day (after 6 days) [13]... 

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