Acetic acid bacteria cultivation media

Gluconobacter growing on GYCM over time (3 to 5 weeks) produces water-soluble brown pigments, which are not seen in the case of any similarly cultivated Acetobacter species. Grown on this medium, Acetobacter will produce clear zones or halos around colonies because the acid being produced will neutralize the CaCOs. Unlike the lactic acid bacteria, acetic acid bacteria are obligate aerobes and so it is necessary to use spread plates. 

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