Accuracy evaluation image analysis

More recently, image analysis has been employed to identify fibers and measure structural characteristics in nonwovens. However, the accuracy and indeed the limitations of these techniques have not been verified. A reliable evaluation of the algorithm requires samples with known characteristics. To accommodate this, a simulation scheme that generates specific nonwoven structures (images) has been developed. 

It is clear that mass spectrometry imaging has great potential as a comprehensive analysis technique for endogenous peptides, particularly with a hardware configuration as evaluated in this chapter, where MALDI produced ions are analyzed in an ion trap - Orbitrap hybrid instrumentation. A single experiment can provide high mass accuracy data in combination with the spatial distribution of peptides in the tissue sample. With MS/MS experiments the molecular identity (accurate mass measurements complemented with MS" sequence data) can be confirmed firom a single or few scans only and from a few laser shots in total. 

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