Acclimation procedure

It is important that the experimental design takes into account the close relationship of fish and their water environment. The data collected is only as good as the water quality, control of water quality throughout the experiment, the match of the water quality with the species under investigation and the acclimation procedure to the desired experimental conditions. Much like the description of analytical methods, water quality conditions should be reported to ensure reproducibility. Of methods employed for studies in fish, these are the most often overlooked. 

Following dialysis and treatment by SEC, the sample extracts were solvent exchanged into sterile DMSO. Subsequently, four rainbow trout Oncorhynchus mykiss [RBT]) were placed in each of seven tanks (each tank is considered as a treatment and a replicate is an individual fish within a tank) in 18 °C well water (280 mg hardness as CaCOs) using flow-through conditions. RBT were fed once daily throughout the study. Following a 48 hour acclimation, RBT were injected interperitoneally with 100 pL of a 1 1 mixture of an SPMD extract or appropriate controls in DMSO or corn oil. Controls included non-deployed SPMD extracts, SEC blanks, and DMSO blanks. The same injection procedure was repeated 6 days later. RBT were sacrificed 11 days after initial exposure to the extracts, and the plasma, liver, gills, and brain were immediately removed from each fish and maintained at -80 °C until assayed. 

Statements on which regulatory guidelines, such as 21 CFR 58 for FDA GLP compliance, will be followed and on Animal Care Committee protocol review Information on the drug substance, which commonly includes the test article name or number, identification criteria, physical description, who is responsible for test article characterization, the concentration(s) to be used, recommended storage conditions, inventory maintenance, formulation procedures, reserve samples, retention samples, analyses for content and homogeneity (if necessary), disposition, and safety precautions Information on the animal species to be studied, animal husbandry procedures, such as housing, food, water, contaminants, environment conditions, acclimation, and justification of selection... 

Test species Information on the animal species to be studied, animal husbandry procedures such as housing, food, water, contaminants, environmental conditions, acclimation, and justification of selection... 

Because individuals of E. inexpectatus often fled and failed to respond when confronted by the experimenter s hand, applicators were tied to the ends of 1 m wire rods which were held at the opposite end by the experimenter during trials. This procedure might also be helpful with fasciatus and other easily frightened species. We have successfully used wooden applicators to introduce stimuli to long-term captive E. laticeps thoroughly acclimated to laboratory conditions and accustomed to the daily introduction of human hands into their home cages. Other techniques will be required to study more timid species. 

Kassongo J, Togo CA. Performance improvement of whey-driven microbial fuel cells by acclimation of indigenous anodophilic microbes. Afr J Biotechnol 2011 10 7846-7852. Kim JR, Min B, Logan BE. Evaluation of procedures to acclimate a microbial fuel cell for electricity production. Appl Microbiol Biotechnol 2005 68 23-30. 

HD values were synthesized by controlled acetylation of commercial PVA (HD = 99%) and submitted to biodegradation tests in aqueous medium, mature compost and soil by using respirometric procedures [121]. Reacetylated PVA samples characterized by HD of between 25 and 75% underwent extensive mineiahzation when buried in solid media, while PVA (HD = 99%) showed recalcitrance to biodegradation under those conditions. An opposite trend was observed in aqueous solution, in the presence of PVA-acclimated microorganisms. In... 

Progressive acclimation to experimental procedures is usually observed in animal models, and haemodynamic parameters are most sensitive to such environmental interferences. When selecting the optimal study design, this progressive change in baseline conditions needs to be considered. In this context, a dose... 

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