Acclaim Mixed-Mode WCX

Analogously, the Acclaim Mixed-Mode WCX-1 column combines both hydrophobic and cation-exchange characteristics. The packing material consists of an alkyl chain with a terminal carboxyl group, so that its selectivity can be... 

Figure 6.81 Selectivity adjustment by changing (a) ionic strength, (b) pH, or (c) organic solvent content in the mobile phase on Acclaim Mixed-Mode WCX-1, 5 pm. Column dimensions ...

In comparison to regular C8 or C18 columns. Acclaim Mixed-Mode WCX-1 exhibits a different elution order for the three model compounds imder the same chromatographic conditions. As shown in Figure 6.82, benzoic add and benzylamine are not separated on Acclaim C8, while naphthalene is very strongly retained. In addition, Acclaim Mixed-Mode WCX-1 provides the selectivity... 

Figure 6.82 Selecdvity comparison between Acclaim Mixed-Mode WCX-1, 5 pm and Acclaim 120 C8,5 pm. Column dimensions 150 mm x4.6 mm i.d. column temperature 30 °C eluent MeCN/IOmmol/L sodium...

Figure 6.83 Separation of antidepressant drugs on Acclaim Mixed-Mode WCX-1,5 pm. Column dimensions 150mmx4.6mm i.d. column temperature SOT eluent MeCN/ 10 mmol/L sodium phosphate buffer, pH 5.2...

The four Acclaim Mixed-Mode columns mentioned in Section 6.7.2 - Acclaim Mixed-Mode WAX-1, Acclaim Mixed-Mode WCX-1, Acclaim Trinity PI, and Acclaim Trinity P2 - can also be used under HILIC conditions. Acclaim Mixed-Mode WAX-1 and Acclaim Mixed-Mode WCX-1 are based on an alkyl chain with terminal ion-exchange functional groups. Because of this column chemistry, both columns offer multiple retention mechanisms, including ion exchange, reversed phase, and HILIC. As an example. Figure 7.7 demonstrates that in highly organic mobile phase. Acclaim Mixed-Mode WCX-1 can serve as a HILIC column. Hydrophilic analytes such as cytosine elute later than naphthalene as a hydrophobic neutral analyte. 

Figure 7.7 RP mode versus HILIC mode on Acclaim Mixed-Mode WCX-1. Column dimensions 1S0mmx4.6mm i.d. column temperature 30°C eluent MeCN/NH40Ac, pH 5 (5 mmol/L total), 95 5 v/v for HILIC mode...

The cationic melamine can be separated on Acclaim Mixed-Mode WCX-1 (see Section 6.7.2) using an ammonium acetate buffer as the eluent. This column features a mixed-mode silica-based packing material that incorporates... 

Dionex Corporation (2009) Dionex Application Note Number 221 Rapid Determination of Melamine in Liquid Milk and Milk Powder by HPLC on the Acclaim Mixed-Mode WCX-1 Column with UV Detection. Dionex Corporation (now part of Thermo Fisher Scientific), Suimyvale, CA, USA. 

Mixed-Mode WCX-1 [68] (Thermo Fisher Scientific, Waltham, MA, USA). The Acclaim Mixed-Mode WAX-1 column combines both hydrophobic and anion-exchange characteristics, which facilitates selectivity adjustment by changing mobile-phase ionic strength, pH, or organic modifier, either independently or concurrently. 

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