Acclaim Mixed-Mode HILIC

Acclaim HILIC-10 is similar to Acclaim Mixed-Mode WAX-1. This relatively new column is also based on high-purity spherical silica covalently modified with a proprietary hydrophilic layer. It is suited for use in a broad range of applications including the separation of hydrophilic drugs and their metabolites. Acclaim Mixed-Mode HILIC-1 is a stationary phase that combines both RP and HILIC properties. Unlike traditional RP or HILIC columns, this packing material features a long alkyl chain with a diol functionality at the terminal end. 

Figure 7.8 Comparison of the chromatographic behavior between Acclaim Mixed-Mode HILIC-1 and LiChrosorb Diol. Separator columns Acclaim Mixed-Mode HILIC-1,5 pm ( ) and LiChrosorb Diol, 5 pm ( ) column...

The four Acclaim Mixed-Mode columns mentioned in Section 6.7.2 - Acclaim Mixed-Mode WAX-1, Acclaim Mixed-Mode WCX-1, Acclaim Trinity PI, and Acclaim Trinity P2 - can also be used under HILIC conditions. Acclaim Mixed-Mode WAX-1 and Acclaim Mixed-Mode WCX-1 are based on an alkyl chain with terminal ion-exchange functional groups. Because of this column chemistry, both columns offer multiple retention mechanisms, including ion exchange, reversed phase, and HILIC. As an example. Figure 7.7 demonstrates that in highly organic mobile phase. Acclaim Mixed-Mode WCX-1 can serve as a HILIC column. Hydrophilic analytes such as cytosine elute later than naphthalene as a hydrophobic neutral analyte. 

Figure 7.7 RP mode versus HILIC mode on Acclaim Mixed-Mode WCX-1. Column dimensions 1S0mmx4.6mm i.d. column temperature 30°C eluent MeCN/NH40Ac, pH 5 (5 mmol/L total), 95 5 v/v for HILIC mode...

Table 7.6 summarizes the structural and technical properties of all currently available Acclaim Mixed-Mode columns that can be used under HILIC conditions. 

Figure 7.16 Separation of selected amino acids under HILIC conditions on Acclaim Mixed-Mode WAX-1. Column dimensions 150mmx4.16mm i.d., 5pm column temperature 30 °C eluent 75 25 (vAr) MeCN/25mmol/L...

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