A new model acid-base cluster hypothesis

This device should be common to all Fj s, and in fact these clusters are found in the homologous sequences in the subunits of MF], CF, and EFj. The acidic cluster is composed of Asp-Glu-Leu-Ser-Glu-Glu-Asp (residues no. 381-387 of EFj) and the basic cluster of Arg-Ala-Lys-Ile-X-Arg (no. 393-399) (Fig. 5.4) [31]. There are other small clusters which are also homologous in F/s, but the clusters mentioned above are located very close to the Rossmann fold, as shown in Fig. 5.6. There are a few clusters in subunits a and b, which may transfer energy of H flux. The invariant Arg-41 of subunit c may become a candidate of the basic cluster if c subunits form an oligomer. Intracistronic mapping of the defective site of the 12y8 subunit mutants in the domain II (from residue no. 288 to the C-terminus) revealed that many of 

Acid base cluster hypothesis in proton motive ATP synthesis 

Primary Homology in the acid base cluster. Mutation in the area should cause inactivation. 

Tertiary ATP-binding should cause stabilization of F] and j8 because of 3 ionic bonds. 

Quaternary Narrow area of contact in /8 to receive H flux from pQ-y. 

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