A Case Study on Neu5Aca2-3Gaipi-4Glc

As an example of the use of some of the techniques described above in the conformational analysis of oligosaccharides, a case study of the solution properties of the trisaccharide Neu5Aca2-3Gaipi-4Glc will be described. 

The first step in the conformational analysis of the oligosaccharide is the determination of complete proton and carbon resonance assignments. These have been reported previously for this particular trisaccharide [80], In systems with unknown assignments, the presence of uniform C-enrichment permits the application of conventional HCCH-COSY and HCCH-TOCSY experiments [81, 82], which invariably give complete resonance assignments in an efficient manner. 

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