SEARCH Articles Figures Tables A direct ELISA linked ICP-MS assay of 3xFLAG-BAP . In this assay, serial dilutions of 3xFLAG-BAP over a concentration range of 0.01-1000 ng mL were incubated overnight at 4 C in the wells of a Maxisorp plate. Negative controls consisted of PBS without protein. The wells were then blocked for 1 h with 3 BSA 1 x PBS buffer at room temperature, washed, and exposed to anti-FLAG-Eu antibodies for 2 h in a shaken incubation. The wells were washed, dried, and the residue dissolved in 10 HCI spiked with 1 ppb Ho and 1 ppb Ir prior to sampling into the ICP-MS. [Publishers Imprint]