SEARCH Articles Figures Tables A convenient scheme for determining inhibitor modality in 96-well format. In this scheme the substrate concentration is varied from 10-fold to 0.08-fold the KM value, representing 91 to 7 saturation, and the inhibitor is evaluated at four discrete concentrations of 0-, 1 3-, 1-, and 3-fold the IC50 value . Each combination of substrate and inhibitor is evaluated in triplicate. [Publishers Imprint]