Zinc pheophytin

Jones, I.D., White, R.C., Gibbs, E., and Butler, L.S. 1977. Estimation of zinc pheophytins, chlorophylls and pheophytins in mixtures in diethyl ether or 80% acetone by spectrophotometry and fluorometry. J. Agric. Food Chem. 25 146-149. 

Fig. 2.132. Chromatogram of spinach, stored frozen until analysis by HPLC (A) and after acidifying the same pigment extract with 0.2ml M HC1 per 10 ml extract and exposure to air and light for 15 h at 20°C (B). Zinc-phtalocyanine was used as an internal standard (IS). Peak identification 1 = chlorophyll-b 2 = chlorophyll-a x = unknown degradation product 3 = IS 4 = pheophytin-b 5 = pheophytin-a 6 = chlorophyll-b 7 = chlorophyll-a 8 = pheophytin-b 9 = pheophytin-a. Reprinted with permission from T. Bohn et al. [303].

Schwartz, S.J. 1984. High performance liquid chromatography of zinc and copper pheophytins. J. Liq. Chromatogr. 7 1673-1683. 

Chlorophyll a and b, pheophytin a, pyropheophytin a, and the zinc complexes of pyropheophytin a and pheophytin a were isolated fix)m ftesh and frozen processed pea presamples [712]. A normal-phase separation using a silica column (A = 658 nm) and a 22-min complex 98.3/1.7- 97/3 hexane/IPA gradient generated excellent resolution and peak shapes. 

Fig. 3 Absorption spectra of some of the chromophores from Figs. 1 and 2. Upper part some natural chromophores. Pheo a stands for pheophytin a, the free base of Chi a after replacement of the magnesium ion by two protons Lower part some synthetic chromophores dark green trace - nickel tetrasulfonated phthalocyanine (PcS4) dissolved in a water DMSO mixture (note the shoulders at 640 and 600 nm due to dimers and H-aggregates, respectively) magenta trace - meso-tetratolyl-porphyrin cyan trace - zinc tetratoljd-porphyrin. Note the sharp 420 nm (Soret) bands of the porphyrins and their very low visible absorptions (the Q bands) in comparison to Chls and phthalocyanines.

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