Zeatin-9-glucoside

Fig. 3. Reverse phase HPLC of a mixture of naturally-occurring cytokinins. Column 150x4.6 mm 5 p.m Spherisorb ODS-2. Mobile phase 30 min, 5-20% acetonitrile in water (pH 7.0 with triethylammonium bicarbonate). Flow rate 2 ml min. Detector absorbance monitor at 265 nm. Sample (1) adenine, (2) adenosine, (3) zeatin-7-glucoside, (4) zeatin-9-glucoside. (5) zeatin-O-glucoside, (6) zeatin, (7) dihydrozeatin-O-glucoside, (8) dihydrozeatin, (9) dihydrozeatin riboside-O-glucoside, (10) zeatin riboside, (11) dihydrozeatin riboside [6].

Zeatin riboside Benzyl adenine Zeatin-7-glucoside Napthaleneacetic acid Sodium docedyl sulphate Polyacrylamide gel electrophoresis Isoelectric focusing... 

This enzyme [EC 2.4.1.118], also known as UDP-glucose-zeatin 7-glucosyltransferase, catalyzes the reaction of UDP-glucose with an A -alkylaminopurine to produce UDP and an A -alkylaminopurine-V-jS-D-glucoside. The enzyme can act on a number of -substituted adenine derivatives (e.g., zeatin and A -benzylaminopurine). However, it has been reported that A -benzyladenme does not serve as a substrate. Depending on the substrate, a 9-/3-D-glucoside may be the product formed. 

H) Dihydrozeatin formation The double bond of the tZ side chain could be enzymatically reduced by zeatin reductase, an enzyme characterized from immature seeds of P. vulgaris, resulting in DZ.429 Zeatin reductase uses tZ as a substrate, but not cZ, tZR, iP, or tZ O-glucoside, and requires NADPH as a cofactor (Figures 15 and 18).429 The responsible genes for this reaction have not yet been identified. 

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