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YM agar

Enzyme Preparation. A crude enzyme mixture was prepared from the fermentate of Candida rugosa (American Type Culture Collection, ATCC No. 14830. The rugosa was revived and cultivated in YM Agar slants at 24°C for one week. The growth on one YM Agar slant was transferred aseptically to a two-liter flask containing 400 ml sterilized fermentation medium consisting of 2.0% defatted soyflour,... [Pg.371]

The yeast strains used were D. hansenii CCMI941, B stipitis CBS 5773, and K. marxianus CBS 6556, obtained from the National Collection of Yeasts Cultures (UK), as NCYC 2597. The strains were maintained on YM agar slants containing 20 g 1 glucose, 3 g 1 yeast extract, 3 g l malt extract, 5 g 1 peptone, and 20 g F agar. [Pg.628]

Microscopic —Oval-shaped vegetative cells on YM agar — Multilateral budding —(1-4) Smooth, round-shaped ascospores on YM agar — Mature club-shaped asci persistent —Rudimentary (simple) pseudomycelium on com meal agar... [Pg.97]

F. Yeast Extract-Malt Extract (YM) Agar YM agar is used to demonstrate ascospore formation in Zygosaccharomyces and, following vitamin supplementation (below), Dekkera sp. YM agar is commercially available from various supply houses and their distributors. Prepare according to manufacturer s recommendations. Sterilize by autoclaving. [Pg.100]

G. YM (Vitamin Supplemented) Dekkera sp. may be sporulated on vitamin-supplemented YM agar (van der Walt and van Kerken, 1961). The components and their final concentration (in sporulation medium) are summarized below. Because some components are present at microgram concentrations, its necessary to prepare several-fold stock solutions which are appropriately diluted during media preparation. [Pg.100]

Sterile filter housing and membrane Young culture of yeast on YM agar... [Pg.102]

Colonies developing are then transferred to either wort or YM agar to demonstrate sporulation. Formation of club-shaped asci after 6 days is considered a presumptive identification. [Pg.109]

Potato dextrose agar Tween-80 Tryptone WL-nutrient agar WL-differential agar Wort agar Yeast carbon base Yeast extract YM agar YM broth... [Pg.175]

See other pages where YM agar is mentioned: [Pg.276]    [Pg.199]    [Pg.294]    [Pg.294]    [Pg.294]    [Pg.297]    [Pg.74]    [Pg.410]    [Pg.410]    [Pg.72]    [Pg.76]    [Pg.76]    [Pg.79]    [Pg.72]    [Pg.76]    [Pg.76]    [Pg.79]   
See also in sourсe #XX -- [ Pg.72 , Pg.76 , Pg.79 , Pg.93 , Pg.94 ]

See also in sourсe #XX -- [ Pg.72 , Pg.76 , Pg.79 , Pg.93 , Pg.94 ]



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