Yeast n-Hybrid System

Fig. 7.1. Different yeast n-hybrid systems that have been developed to study protein-protein, protein-DNA, protein-RNA, and protein - small molecule interactions. A. In the original version of the Y2H system, transcriptional activation of the reporter gene is reconstituted by recruitment of the activation domain (AD) to the promoter region through direct interaction of protein X and Y, since protein X is fused to a DNA-binding domain (DBD) and protein Y is fused to the AD [1], B. In the Y1 H assay, the AD is fused directly to the DBD [109]. This assay can be used to screen either DBDs that can bind to a specific DNA sequence or the in vivo binding site for a given DBD. C.

Kamada, S., H. Kusano, H. Fujita, M. Ohtsu, R.C. Koya, N. Kuzumaki, and Y. Tsujimoto. 1998. A cloning method for caspase substrates that uses the yeast two-hybrid system cloning of the antiapoptotic gene gelsolin. Proc Natl Acad Sci USA. 95 8532—7. 

ARVD vs. CPVT Yeast two-hybrid system N/A N/A f CPVT, ARVD N/A (Tiso and Thiene 2001)... 

Vidal, M. and Legrain, P. (1999) Yeast forward and reverse n -hybrid systems. Nucleic Acids Res. 27, 919-929. 

Cervantes S, Gonzalez-Duarte R, Marfany G. Homodimerization of presenUin N-terminal fragments is affected by mntations linked to Alzheimer s disease. FEBS Lett. 2001 505 81-86. Thaminy S, Auerbach D, Arnoldo A, Stagljar I. Identification of novel ErbB3-interacting factors using the split-ubiquitin membrane yeast two-hybrid system. Genome Res. 2003 13 1744-1753. 

Piganeau N, Schauer UE, Schroeder R. A yeast RNA-hybrid system for the detection of RNA-RNA interactions in vivo. Rna 2006 12 177-184. 

Bernstein DS, Buter N, Smmpf C, Wickens M. Analyzing mRNA-protein complexes using a yeast three-hybrid system. Methods 2002 26 123-141. 

Yeast artificial chromosome 1111 Yeast cell surface display 1112 Yeast expression vector 1028 Yeast-2-hybrid system 1161 Yeast signal trap 109 Yttrium-90 Zevalin 456, 480 Yttrium-DOTALA.N 518 Yttrium-DOTATATE 518 Yttrium-DOTATOC 516... 

Hunter, N., Chambers, S.R., Louis, E.J., Borts, R.H. (1996). The mismatch repair system contributes to meiotic sterility in an interspecific yeast hybrid. EMBO J. 15, 1726-1733. 

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