Xenobiotics biomarker identification

After xenobiotic/toxin exposure, differentially expressed proteins are identified by the comparison of SELDI spectra from control and treated samples. By combining groupwise statistics with N-fold regulations, single biomarkers (m/z) can be selected. As to be expected from the complexity of the proteome, in many cases no single marker will be able to discriminate between the groups. Rather, a complex pattern of multiple markers will be acquired (Figure 8). Discovery of such markers/pattems can be successful by application of multivariate statistics methods on the data set. However, for the identification of specific protein expression patterns bioinformatics tools are... 

Human data for various cardiotoxic scenarios are unavailable. The means of prompt identification of the particular xenobiotic causing poisoning remains an urgent task. Progress in metabolomics and the identification biomarkers that result from metabolic changes caused by the presence of xenobiotics will enable the development of chip-based rapid-responding assaying devices. 

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