Worthington Biochemical Corporation

C. Worthington, Editor, Worthington Enzyme Manual (1988), Worthington Biochemical Corporation (Freehold, NJ), pp. 288-291. Properties and analysis of tyrosinase. http //www.worthingtonbiochem.eom/manual/P/TY.html... 

Salt-free trypsin (Worthington Biochemical Corporation Lot No. 688SF) unoxidized, and oxidized as described in the text, were hydrolyzed by constant-boiling hydrochloric acid for 20 hr at 105°C. Except for the oxidation step, the samples were treated identically. The oxidized sample posse.ssed 3% of the original activity. The hydrolyzates were analyzed for amino acids using the Automatic Amino Acid Analyzer (Beckman-Spinco) developed by Spaokman et al. (1958). Ninety-nine and 97% of the applied nitrogen was recovered in the case of unoxidized and oxidized trypsin hydrolyzates respectively. 

Worthington Enzyme Manual Enzymes, Enzyme Reagents, Related Biochemicals," p. 39. Worthington Biochemical Corporation, Freehold, New Jersey, 1972. 

Worthington, C. E. (1972), Enzymatic assay of urease from Jack Beans (E.C.3.5.1.5). In Worthington Enzyme Manual (pp. 146-148). Freehold, NJ Worthington Biochemical Corporation. 

Worthington Biochemical Corporation, http //www.worthington-biochera.com. (catalog information on enzymes). 4/1/07. 

The final nucleotide concentrations of the seunples were o.o8 M as determined by spectrophotometrlc methods. (The pH was adjusted with a Radiometer PH meter (PHM 26) In conjunction with an Ingold type 4o5-M 3/2 combined electrode.) Bovine pancreatic rlbonuclease A (phosphate free) was obtained from Worthington Biochemical Corporation (Freehold,... 

Uric acid was isolated from urine by adsorption on Darco-G60 at pH 4.2 overnight at 4 C, eluted with hot O.IN NaOH, and repeatedly recrystallized from Li2C03 solution. Uric acid analyses were performed by the differential spectrophotometric method [23] employing purified uricase purchased from Worthington Biochemical Corporation. 

Use best quaUty chromatographically pure bacterial (E. coli) alkaline phosphatase such as that obtainable from the Worthington Biochemical Corporation, Freehold, New Jersey, USA. The stock enzyme (ca. 20 units/mg) should be stored at near to 0 C in a refrigerator. Transfer sufficient enzyme solution with a hypodermic syringe into 100 ml of distilled water to give a solution ... 

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