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Whole-column imaging

Figure 3 Instrument setup of whole-column imaging detection for CIEF. Absorption or refractive index gradient mode with camera (I) placed in the direction of illuminated light fluorescence mode with camera (II) placed vertically to the direction of illuminated light. (Reproduced from Mao, Q., Pawliszyn, J J. Biochem. Bio-phys. Meth. 39, 1999, 93-110, with permission of Elsevier Science Publishers.)... Figure 3 Instrument setup of whole-column imaging detection for CIEF. Absorption or refractive index gradient mode with camera (I) placed in the direction of illuminated light fluorescence mode with camera (II) placed vertically to the direction of illuminated light. (Reproduced from Mao, Q., Pawliszyn, J J. Biochem. Bio-phys. Meth. 39, 1999, 93-110, with permission of Elsevier Science Publishers.)...
Figure 8 Illustration of whole-column imaging miniaturized capillary isoelectric focusing instruments with a LED as light source. A 1.2-cm capillary is used as a separation column for focusing. The outside polyimide coating of the capillary is removed, and the inside surface of capillary is coated with non-cross-linked polyacrylamide to eliminate electroosmotic flow. The two ends of the capillary are connected with inlet and outlet capillaries (with the same i.d. and o.d. as the separation capillary) by two pieces of porous hollow fiber. Two glass tubes are used as electrolyte tanks and glued directly on the glass slide by epoxy glue. The two pieces of hollow fiber are in the electrolyte tanks. The length of the capillary between the two electrolyte tanks is about 0.9 mm. Figure 8 Illustration of whole-column imaging miniaturized capillary isoelectric focusing instruments with a LED as light source. A 1.2-cm capillary is used as a separation column for focusing. The outside polyimide coating of the capillary is removed, and the inside surface of capillary is coated with non-cross-linked polyacrylamide to eliminate electroosmotic flow. The two ends of the capillary are connected with inlet and outlet capillaries (with the same i.d. and o.d. as the separation capillary) by two pieces of porous hollow fiber. Two glass tubes are used as electrolyte tanks and glued directly on the glass slide by epoxy glue. The two pieces of hollow fiber are in the electrolyte tanks. The length of the capillary between the two electrolyte tanks is about 0.9 mm.
Fang X, Tragas C, Wu J, et al. (1998). Recent developments in capillary isoelectric focusing with whole-column imaging detection. Electrophoresis. 19 2290-2295. [Pg.503]

Liu, Z. and Pawliszyn, J., Coupling of solid-phase microextraction and capillary isoelectric focusing with laser-induced fluorescence whole column imaging detection for protein analysis. Analytical Chemistry, 77,165, 2005. [Pg.822]

Mao Q and Pawliszyn J (1999) Capillary isoelectric focusing with whole column imaging detection for analysis of proteins and peptides. Journal of Biochemical and Biophysical Methods 39 93-110. [Pg.3516]

See other pages where Whole-column imaging is mentioned: [Pg.190]    [Pg.58]    [Pg.59]    [Pg.61]    [Pg.61]    [Pg.292]    [Pg.293]    [Pg.671]    [Pg.477]    [Pg.84]    [Pg.307]    [Pg.519]    [Pg.528]    [Pg.1264]    [Pg.1731]    [Pg.1731]    [Pg.220]    [Pg.221]   
See also in sourсe #XX -- [ Pg.307 ]



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