Whiting reaction, modifie

Boiling an egg causes the transparent and gelatinous albumen ( egg white ) to modify chemically, causing it to become a white, opaque solid. Like all chemical reactions,... 

Modified Whiting reaction s. 11, 111 Arenes from quinones... 

Modifying the reaction medium to involve liquid ammonia with metallic lithium, f-butyl alcohol, and white phosphorus, to which is added the haloalkane, is reported to provide the primary alkylphos-phine derived from the haloalkane.19 Similar results are reported for the reaction of red phosphorus with sodium acetylides20 and by treatment of red phosphorus with sodium metal in an organic medium followed by the addition of two equivalents of f-butyl alcohol and the haloalkane.21 The latter approach is noteworthy in that moderate yields (45%) are obtained for primary phosphines derived from secondary haloalkanes (Figure 2.6). Mixtures of tertiary phosphines bearing one or two acetylenic linkages are produced in low yield ( 15%) by the reaction of lithium acetylides with white phosphorus in liquid ammonia followed by addition of a haloalkane.22... 

Zhao et al. prepared magnetite (FesO nanoparticles modified with electroactive Prussian Blue [44]. These modified NPs were drop-cast onto glassy-carbon electrodes. They observed the redox processes commonly observed for PB (similar to that seen in Figure 4.8), and also demonstrated that the Prussian White material produced by PB reduction at 0.2 V served as an electrocatalyst for Fi202 reduction. They also prepared LbL films in which PB NPs and glucose oxidase were alternated between PD DA layers [99]. These were demonstrated to act as electrocatalysts for Fi202 reduction. Based on the ability to sense the product of the enzymatic reaction, these structures were shown to act as glucose sensors. 

Dilute IMMEDIATELY with 40 p, TE Buffer (10 mM Tris, pH 8.0,1 mM EDTA) and either transform IMMEDIATELY or freeze the reaction until you are ready to transform. coli with a transformation efficiency in excess of 1 x 10 are recommended and if the vector has been modified for blue-white screening ensure that an appropriate E. coli host strain is used 5 pi of the diluted reaction should give tens to hundreds of colonies per well of a 24-well plate. 

---