When Is a Difference Real

MS data points were scrutinized to determine if there were any significant correlations between the magnitude of the SD and the following factors  

Peptide size (i.e., the theoretical number of exchangeable backbone amides in each peptide) 

The magnitude of deuteration that is experimentally observed (expressed as the absolute magnitude of deuterium incorporation, Da) 

An extension of point 3, where we express the magnitude of the level of difference in exchange as a fraction of the maximum HX possible (i.e., 100%) for the given peptide, which was obtained by dividing the D AM. )) data by the maximum theoretical number of exchangeable amides possible for the given peptide 

1 Criteria for Assessing the Presence of a Difference in HX-MS Comparability Experiments 

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