Wheat germ lipase

Recently, an interesting example of the enzymatic kinetic resolution of a-acetoxyamide 8 was demonstrated using native wheat germ lipase and immobilized lipase PS (AMANO) (Scheme 5.6). 

Muzzarelli RAA (1997) Human enzymatic activities related to the therapeutic administration of chitin derivatives. Cell Mol Life Sci 53 131-140 Muzzarelli RAA, Xia W, Tomasetti M and Ilari P (1995) Depolymerization of chitosan and substituted chitosans with the aid of a wheat germ lipase preparation. Enz Microbial Tech 17 541-545... 

Wheat germ lipase was purchased from the Sigma Chemical Company. 

PLLA, PDLA, PLDLA Rod diam. 2 mm, 1 10mm Mn 2.2 PBS (pH 7.2) containing either porcine liver esterase (PLE), wheat germ lipase (WGL), porcine pancreatic lipase (PPL), or Rhizopus delemar Wpase (RDL) 37 Wt. loss Degradation rate RDL>PPL>PLE>WGL>PBS Copolymers degrade faster than homopolymers 23... 

Trioleoylglycerol (triolein) and olive oil are commonly used substrates, and stable emulsions may be readily obtained for use as substrate preparations. Enzyme activity can be monitored by a pH stat or analysis of released fatty acids (Brockerhoff and Jensen, 1974). The sensitivity of low-level activities may be increased with radioactive substrates. Tributyrin is commonly used because of its ease of dispersion in water, but it cannot be assumed that tributyrin hydrolysis is necessarily a measurement of true lipase activity. Triacetin is water-soluble, and although often used for lipase determinations this practice is not recommended. (A major commercial enzyme supplier markets wheat germ lipase but states on the containers that the preparation will not hydrolyze olive oil—it is, however, active on triacetin.)... 

Monoacylation by use of lipase/trifluoroethyl butanoate systems occurred predominantly at the 2-position of methyl 6-O-butanoyl-a-D-glucopyranoside and at the 4-posidon of methyl 6-deoxy-a-L-glucopyranoside. Methyl 4,6-O-benzylidene-a- and - D-glucopyranoside were preferentially acetylated at 0-2 and 0-3, respectively, by lipase/vinyl acetate Exposure of the galacturono-6,1-lactone triacetate 32 (see Chapter 16 for its synthesis) to wheat germ lipase gave the diacetate 33 in modest yield. ... 

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