96-well plates with screen filter

The advantages of this screen filter, as cited by the authors, consist of its reusability, its standard 96-well format size, and its ability to be used whenever sample transfer or pipetting is needed. After usage, the screen filter can be easily cleaned by rinsing with water and methanol and additionally, by ultrasonication in water or methanol or other appropriate solution. The filter can be inserted into a plasma storage plate before sample transfer by the Tomtec Quadra used by the authors for automation. 

ELISAs (enzyme-linked immunoabsorbent assays) are another common framework used for drug screening. An enzyme-linked-antibody takes the place of a ligand, whose receptor is bound to a plate or filter. The mixture of drug and enzyme-linked antibody is incubated in the well with the receptor. After a series of washes to remove unbound material, the substrate for the enzyme is added to the well. A common enzyme/substrate pair is alkaline phosphatase and pNPP ( -nitrophenyl phosphate), which results in a yellow color. Another... 

Figure 2. Screening of a pea cDNA library for CA immunopositive clones. Nitrocellulose filters prepared from 86 mm plates containing approximately 10,000 recombinant plaques were incubated with the CA antisera. Specifically bound antibody was detected with horseradish peroxidase conjugated goat anti-rabbit IgG. A positive signal on a primary plate is shown (A) as well as the tertiary stage of plaque purification (B). A control filter with no positive signals is also presented (C).

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