WALTZ-16 decoupling

Carbon-13 and Edited DEPT Spectra with Waltz-16 Decoupling... 

Fig. 1. Increases in steady-state temperature from WALTZ-16 decoupling of saline solutions containing 10% D2O. (Figure from ref. 19, Copyright 1988 by Academic Press, reproduced by permission of the publisher.)...

Fig. 23.16. C spectra at 25°C of KIF reassembled in vitro from native prekeratin labeled with [l- C]Gly, acquired at 62.98 MHz. (A) Spectrum obtained under conditions of low-level WALTZ-16 decoupling with Overhauser enhancement. (B) Spectrum obtained with high-power coherent decoupling with NOE. About 200 Hz of line broadening was applied. Each spectrum is the average of 12600 acquisitions. Recycle times of 5 s were used.

Fig. 23.17. C spectra at 25°C and 62.98 MHz of isolated KIF. (A) A WALTZ-16 decoupled spectrum of a sample incorporating [2- C]Gly. The a-carbon resonance is at ca. 42 ppm. S and G mark the locations of the serine and glycine a-carbon peaks. (B) A spectrum of the same sample taken under high-power coherent decoupling. (C) Spectrum of a sample incorporating l-[l- C]leucine, WALTZ decoupling. (D) A high-power decoupled spectrum of the same sample as in (C). The upheld aliphatic regions of spectra C and D serve essentially as natural abundance comparisons for the analogous regions of spectra A and B of the a-labeled sample. Lorentzian broadening of 20 Hz is used in each spectrum. Each spectrum is the result of ca. 16,000 scans.

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