Vivo Drug Screening

Methods and approaches that can simultaneously determine mixtures of drug candidates provide a powerful way to select an optimal drug candidate within a specific therapeutic area or a targeted class of compounds. Using relatively simple isolation and chromatography conditions, LC/MS/MS approaches are an effective way to evaluate new lead compounds for subsequent development. Consequently, 

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In general, standard methods applicable to a vast majority of compounds of interest to ensure throughput capabilities are critical for LC/MS screens. Although not optimized for specificity, standard conditions provide a systemic measure of control. This control results in data that has high quality, reliability, and comparability. With a strategic selection of compounds that have similar molecular weights, structural features, and chromatographic properties, the detection selectivity and precision are satisfactory for this particular type of analysis. 

Once an LC/MS-based screen is developed and locked in for use (i.e., established as the standard method for subsequent analyses), the object is to arrive at the most promising lead compounds within a series quickly without the traditional, rigorous testing and analysis of each candidate separately. The reward is a significant saving in time and resources. The inherent risk is devising a discriminatory 

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Olah, T. V., McLoughlin, D. A., Gilbert, J. D., The simultaneous determination of mixtures of drug candidates by liquid chromatography/ atmospheric pressure chemical ionisation mass spectrometry as an in-vivo drug screening procedure, Rapid Commun. Mass Spec. 1997, 11, 17—23. 

Qlah, T.V. McLoughlin, D.A. Gilbert, J.D. The Simultaneous Determination of Mixtures of Drug Candidates by Liquid Chromatography/Atmospheric Pressure Chemical Ionization Mass Spectrometry as an In Vivo Drug Screening Procedure, Rapid Commun. Mass Spectrom. 11,17-23 (1997). 

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