Vitro Skin Impedance Guided High-Throughput Screening

IN VITRO SKIN IMPEDANCE GUIDED HIGH-THROUGHPUT SCREENING 

With reference to the design criteria highlighted in the previous section, INSIGHT provides the following advantages over FDCs  

INSIGHT can assay 1000 to 1500 formulations per day, a 100-fold increase over the screening rate obtained by FDCs (46). 

Skin impedance measured in INSIGHT at 24 hours correlates very well with skin inulin permeability at steady states (Fig. 5). This reduces incubation times, as compared to FDCs. 

INSIGHT uses skin impedance to evaluate the efficacy of formulations in increasing skin permeability as against the use of a model permeant. As a result, there is no dependence of the physicochemical properties of the model permeant on the measured efficacy of the formulation. 

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Figure 4 (A) Schematic of in vitro skin impedance guided high-throughput screening tool, INSIGHT. (B) Skin permeability skin impedance correlation for 1.5%wt/vol. menthol (open squares), 1.5%wt/vol. lauric acid (filled triangles), 1.5%wt/vol. Brij 35 (open circles), 1.0%wt/vol. Lauryl sarcosinate (filled circles), 1.0%wt/vol. sorbitan monolaurate (open triangles), and 1.5%wt/vol. benzyldodecyldimethyl ammonium chloride (filled squares) in 1 1 PBS EtOH. Abbreviation PBS EtOH, phosphate buffered saline ethanol.

Figure 5 Plot of conductivity enhancement ratios (ERs) in INSIGHT at 24 hours versus permeability ERs in FDC for 12 enhancer formulations. A strong linear correlation indicates the validity of observations in INSIGHT when compared with those from traditional tools such as FDC. Abbreviations. INSIGHT, in vitro skin impedance guided high-throughput screening FDC, Franz diffusion cell.

VALIDATION OF IN VITRO SKIN IMPEDANCE GUIDED HIGH-THROUGHPUT SCREENING WITH FRANZ DIFFUSION CELLS... 

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