Virus capture

The methods outlined below consist of 1) tube coating with the capture antibody, 2) sample extraction from potato, 3) virus capture in the coated tubes, 4) cDNA synthesis from the captured viruses and 5) PCR amplification of the cDNA. Tube coating requires an overnight incubation, so should be set up on the day prior to extractions. 

Coat 96-tube PCR plates, which are certified DNase- and RNase-free) with 50 iL of virus-capture antibody (1 mg/mL) diluted 1 100... 

The importance of mechanically controlling these structures in Lab-on-a-Chip systems with dielectrophoretic techniques will aid in the understanding of their biological interactions with other subcellular entities. For example, miniature quadrupole traps can be used to determine the crossover frequency of specific proteins and viruses. Capturing viruses at a particular set of... 

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