Velocity, abnormal distribution

We have recently examined the responsiveness to Mg + and MgATP of the subunit aggregates from a superactive form of PRPP synthetase with normal substrate and inhibitor binding properties but an increased maximal reaction velocity. The distribution of forms of the mutant enzyme on sucrose gradient after the dilution and incubation procedures described above was indistinguishable from that of normal PRPP synthetase. In several respects, however, the mutant enzyme behaved in an abnormal fashion. First, under conditions of suboptimal Mg2+... 

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