Vascular proteomics

Translation of Vascular Proteomics into Individualized Therapeutics... 

Handy, D.E., and Loscalzo, J. (2006). Nitric oxide and posttranslational modification of the vascular proteome S-nitrosation of reactive thiols. Arterioscler. Thromb. Vase. Biol. 26, 1207-1214. 

Armexin A1 emerged as a promising antigen for the radiolabeled antibody-based imaging and therapy of cancer [100]. In our laboratory, we use terminal perfusion protocols featuring active esters of biotin for the selective chemical labeling of accessible proteins in vascular structures. Biotinylated proteins are then purified from different organs (collected separately) and are submitted to a comparative proteomic analysis [106]. 

However, NIR specua are distinctive for proteins in the blood vessel wall (specifically collagens and elastin). The ability of NIR specuometry to collect useful spectra in aqueous environments may make it useful for proteomics in vivo. The results of this study suggest that NIR spectroscopy is a potentially useful technique for investigating vascular changes and protein composition associated with AAA in a mouse model of this disease. These results support an expanded study in the future to correlate NIR spectra with chemical compositions and histological features in aortic aneurysm, which is known to often be associated with atherosclerosis. 

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