Variations of Hyperbolic Inhibition in Monosubstrate Reactions

As we have pointed out in Section 6.1, Eq. (6.2) is quite general and describes a number of different inhibition mechanisms, including many bnear and nonbnear inhibitions. A difference between various types of nonlinear mechanisms is made on the basis of the relative magnitudes of a and values (Segel, 1975). 

it is clear that the primary double reciprocal plots of i/Dq versus 1/A represent a valuable general diagnostic tool for the estimation of the type of nonlinear inhibition mechanisms. 

A rate equation for each nonlinear mechanism shown in Fig. 4 can be easily derived from the general rate Fq. (6.2), simply by inserting the proper values for kinetic constants a and fi, and cancehng out identical numerators and 

Note that the rate equation for the hyperbolic noncompetitive inhibitions (Cases 3, 4, and 6) is identical with the general rate Eq. (6.2). 

Mechanism a fX jS Intersection point Coordinates of intersection points 

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