Validation of Isotherm Models

After the adsorption isotherm experiments have been completed, an isotherm equation must be chosen. This equation should fit the experimental data. Often are the experimental data (the experimental adsorption data acquired by the FA method or the perturbation retention times acquired by the PP method) only compared with the ones calculated using the adsorption isotherm parameters acquired from some adsorption isotherm models [131], This is sometimes the only validation done in this field [131], However, the adsorption isotherm parameters should preferably be validated in two step (1) the different isotherm models should be compared using statistical calculations, e.g., an F-test, and (2) by using the parameters to computer simulate elution profiles and then compare them with experimental ones. 

To conclude to which isotherm models the data fits best the values of the residuals should be used in an F-test [132], In paper III an F-test comparing different adsorption isotherm parameters is done and clearly explained. 

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