Utility of HPLC Analysis in Dissolution Testing

In comparison with spectrophotometric methods (e.g., UV), HPLC methodology often provides the following features wide dynamic linear range, improved specificity via separation and increased sensitivity. These features may be used to solve a variety of analytical problems encountered in modern dissolution systems. 

In contrast with UV methods where the linear range is approximately 1-2 orders of magnitude at best, the HPLC method with UV detection typically has a linear range of 3-4 orders of magnitude due to the narrow path length of the detector flow cell. For example, a simple 

One of the most important advantages of HPLC over spectrophoto-metric methods lies in its specificity and selectivity due to its separation capability. Through chromatographic separations, the analytes of interest can be detected and quantified without interference from the typical matrix that includes excipients, antioxidants, preservatives, and dissolution media. Ion-pair HPLC was used to monitor the dissolution of pentamidine from EVA sustained-release film where polymeric matrices could create significant bias if a spectrophotometric method were used. Due to their strong UV absorbance, the antioxidants and preservatives (e.g., BHA, BHT, ascorbic acid and propyl gallate) are often the major 

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