Use of ligand fluorescence to monitor binding reactions

In a two step reaction such as this with only one source of signal change at the isomerization step the observed rate of the reaction is as described by equation 

Determination of kobs the binding of warfarin to human serum albumin 

1 Dilute some of the warfarin solution to SO p.M using the phosphate buffer. 

4 Fill both of the stopped-flow drive syringes with buffer and discharge 5-10 shots of these to wash the observation cell. 

6 Refill the syringes with phosphate buffer and discharge 10 shots and record the signal voltage (buffer baseline). 

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