Use of Dense Cell Suspensions

For organisms that grow poorly in liquid medium, it may be difficult to obtain sufficient quantities of cells cultures may then be grown on the surface of agar plates and the cells removed by scraping. 

Organisms such as actinomycetes may not produce well-suspended growth and shaking, for example, in baffled flasks or in flasks with coiled-wire inserts may be advantageous in partially overcoming this problem. 

For anaerobic bacteria, the same principles apply, except that additional attention must be directed to preparing the cell suspensions. Use of an anaerobic chamber in which cultures can be transferred to tightly capped centrifuge tubes is virtually obligatory, and addition of an anaerobic indicator should be used to ensure that subsequent entrance of oxygen does not take place inadvertently. On account of the inserts, screw-capped Oak Ridge tubes are convenient for centrifugation. 

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