Uridine diphosphate glucose extracts

Stoichiometric quantities of uridine diphosphate glucose were used, in the presence of a transfer enzyme, sucrose synthetase, in the soluble state (extraction given). Coupling with modified D-fructose gave sucroses modified on the D-fructosyl group, on the 1 -3-mmol scale. Thus were prepared l -deoxy-l -fluoro- (59%),98 4 -deoxy-4 -fluoro- (16%), and l -azido-l -deoxy-sucrose (15%).71 6-Deoxy-6-fluoro-D-glucose was isomerized to 6-deoxy-6-fluoro-D-fructose with isomerase, and gave 6 -deoxy-6 -fluoro-sucrose.71... 

Enzymatic synthesis of uridine diphosphate glucose has been observed, but there are no published reports on how the synthesis takes place. However, we do have some indication of how the compound may act as a coenzyme. When incubated with an extract of S. fragilis it is transformed into uridine diphosphate galactose. This would be a biological Walden inversion. Discovery of this reaction has led to the following hypothesis for the mechanism of the conversion of galactose-1-phosphate to glucose-l-phosphate ... 

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